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Citation

Ran Z, Xu J, Liao K, Monroig ?, Navarro JC, Oboh A, Jin M, Zhou Q, Zhou C, Tocher DR & Yan X (2019) Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: characterization of four fatty acyl elongases and a novel desaturase capacity. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1864 (8), pp. 1083-1090. https://doi.org/10.1016/j.bbalip.2019.04.004

Abstract
As an unusual economically important aquaculture species, Sinonovacula constricta possesses high levels of long-chain polyunsaturated fatty acids (LC-PUFA). Previously, our group identified fatty acyl desaturases (Fad) with Δ5 and Δ6 activities in S. constricta, which was the first report of Δ6 Fad in a marine mollusc. Here, we further successfully characterize elongases of very long-chain fatty acids (Elovl) in this important bivalve species, including one Elovl2/5, two Elovl4 isoforms (a and b) and a novel Elovl (c) with Elovl4 activity. In addition, we also determined the desaturation activity of S. constricta Δ6 Fad toward 24:5n-3 to give 24:6n-3, a key intermediate in docosahexaenoic acid (DHA) biosynthesis. Therefore, S. constricta is the first marine mollusc reported to possess all Fad and Elovl activities required for LC-PUFA biosynthesis via the ‘Sprecher pathway’. This finding greatly increases our understanding of LC-PUFA biosynthesis in marine molluscs. Phylogenetic analysis by interrogating six marine molluscan genomes, and previously functionally characterized Elovl and Fad from marine molluscs, suggested that DHA biosynthetic ability was limited to a few species, due to the general lack of Δ4 or Δ6 Fad in most molluscs.

Keywords
docosahexaenoic acid biosynthesis; fatty acyl desaturases; elongation of very long-chain fatty acids protein; Long-chain polyunsaturated fatty acids; Sinonovacula constricta

Journal
Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids: Volume 1864, Issue 8

• Ran Et Al (Razor Clam) REPOSITORIO