I have been a Lecturer in Cell Biology at the 我要吃瓜 since 2014. Prior to that, I was in universities in Birmingham and London working as a post-doctoral researcher. For 4 years, I was also in working in industry, as Senior Scientist for nanoTherics Limited. I completed my PhD in Biochemistry at Imperial College London (1999 - 2003) my BSc in Biotechnology at University College London (1st Class Honours, 1997 - 1999) and my Diploma in Biotechnology at Singapore Polytechnic (1993 -1995).
Outside of work, I am interested in reading, hill-walking and issues relating to the Christian faith, woodland and wildlife management.
I am interested in studying the immune system which consist of a number of biological processes that protects an organism from diseases. I adopted a number of "model" organisms to study this complicated process and these include vertebrates (cells from mice and humans), invertebrates (mainly insects) and plants (garden plants and woodland trees).
Specifically, I am interested in addressing the following questions:
1) How do environmental stressors and/or nutrients modulate immunity in animals?
2) What similarities and differences exist between phagocytes from vertebrates and invertebrates?
3) How does the immune system in plants cope with environmental stressors?
Evaluating the impact of chronic exposure to low dose ionising radiation on immunity
PI: Dr Jenson Lim
Funded by: The Carnegie Trust
–
Article
Low dose γ-radiation induced effects on wax moth (Galleria mellonella) larvae
Copplestone D, Coates CJ & Lim J (2023) Low dose γ-radiation induced effects on wax moth (Galleria mellonella) larvae. Science of The Total Environment, 876, Art. No.: 162742. https://doi.org/10.1016/j.scitotenv.2023.162742
Article
Effect of acute ultraviolet radiation on Galleria mellonella health and immunity
Sabockyte A, McAllister S, Coates CJ & Lim J (2023) Effect of acute ultraviolet radiation on Galleria mellonella health and immunity. Journal of Invertebrate Pathology, p. 107899. https://www.sciencedirect.com/science/article/pii/S0022201123000162; https://doi.org/10.1016/j.jip.2023.107899
Conference Paper (published)
Biomarkers and Ecological indicators for Environmental Radioactivity in Invertebrates
Raines K, Goodman J, Copplestone D & Lim J (2022) Biomarkers and Ecological indicators for Environmental Radioactivity in Invertebrates. In: Biomarkers of Radiation in the Environment. NATO Science for Peace and Security Series A: Chemistry and Biology. NATO Advanced Research Workshop on 'Biomarkers of Radiation in the Environment: Robust Tools for Risk Assessment (BRITE)’, Yerevan, Armenia, 28.11.2017-30.11.2017. Dordrecht, Netherlands: Springer, pp. 245-270. https://doi.org/10.1007/978-94-024-2101-9_15
Article
Lim J (2022) Broad toxicological effects of Per-/Poly- fluoroalkyl substances (PFAS) on the unicellular eukaryote, Tetrahymena pyriformis. Environmental Toxicology and Pharmacology, 95, Art. No.: 103954. https://doi.org/10.1016/j.etap.2022.103954
Article
Lim J, Petaroudi M, Pearce G, Yasak?i V & Unak P (2021) Effects of Fluorodeoxyglucose Conjugated and Unconjugated Iron Oxide Magnetic Nanoparticles on Macrophages: a Pilot Study. Brazilian Archives of Biology and Technology, 64, Art. No.: e21200736. https://doi.org/10.1590/1678-4324-2021200736
Article
Krachler AM, Sirisaengtaksin N, Monteith P, Paine CET, Coates CJ & Lim J (2021) Defective phagocyte association during infection of Galleria mellonella with Yersinia pseudotuberculosis is detrimental to both insect host and microbe. Virulence, 12 (1), pp. 638-353. https://doi.org/10.1080/21505594.2021.1878672
Article
The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid
Coates CJ, Lim J, Harman K, Rowley AF, Griffiths DJ, Emery H & Layton W (2019) The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid. Cell Biology and Toxicology, 35 (3), pp. 219-232. https://doi.org/10.1007/s10565-018-09448-2
Article
Subramanian M, Miaskowski A, Jenkins SI, Lim J & Dobson J (2019) Remote manipulation of magnetic nanoparticles using magnetic field gradient to promote cancer cell death. Applied Physics A, 125 (4), Art. No.: 226. https://doi.org/10.1007/s00339-019-2510-3
Article
Characterizing the Mechanisms of Nonopsonic Uptake of Cryptococci by Macrophages
Lim J, Coates CJ, Seoane PI, Garelnabi M, Taylor-Smith LM, Monteith P, Macleod CL, Escaron CJ, Brown GD, Hall RA & May RC (2018) Characterizing the Mechanisms of Nonopsonic Uptake of Cryptococci by Macrophages. Journal of Immunology, 200 (10), pp. 3539-3546. https://doi.org/10.4049/jimmunol.1700790
Article
Subramanian M, Tyler A, Luther EM, Di Daniel E, Lim J & Dobson J (2017) Oscillating Magnet Array-Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies. Nanomaterials, 7 (2), Art. No.: 28. http://www.mdpi.com/2079-4991/7/2/28; https://doi.org/10.3390/nano7020028
Article
Lim J, Stones DH, Hawley CA, Watson CA & Krachler AM (2014) Multivalent Adhesion Molecule 7 Clusters Act as Signaling Platform for Host Cellular GTPase Activation and Facilitate Epithelial Barrier Dysfunction. PLoS Pathogens, 10 (9), Art. No.: e1004421. https://doi.org/10.1371/journal.ppat.1004421
Article
Subramanian M, Lim J & Dobson J (2013) Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes. PLoS ONE, 8 (7), Art. No.: e69812. https://doi.org/10.1371/journal.pone.0069812
Article
Lim J, Thompson J, May RC, Hotchin NA & Caron E (2013) Regulator of G-Protein Signalling-14 (RGS14) Regulates the Activation of αMβ2 Integrin during Phagocytosis. PLoS ONE, 8 (6), Art. No.: e69163. https://doi.org/10.1371/journal.pone.0069163
Article
Lim J, Clements MA & Dobson J (2012) Delivery of short interfering ribonucleic acid-complexed magnetic nanoparticles in an oscillating field occurs via caveolae-mediated endocytosis. PLoS ONE, 7 (12), Art. No.: e51350. https://doi.org/10.1371/journal.pone.0051350
Article
Signalling mechanisms of the leukocyte integrin αMβ2: Current and future perspectives
Lim J & Hotchin NA (2012) Signalling mechanisms of the leukocyte integrin αMβ2: Current and future perspectives. Biology of the Cell, 104 (11), pp. 631-640. https://doi.org/10.1111/boc.201200013
Article
Lim J & Dobson J (2012) Improved transfection of HUVEC and MEF cells using DNA complexes with magnetic nanoparticles in an oscillating field. Journal of Genetics, 91 (2), pp. 223-227. https://doi.org/10.1007/s12041-012-0164-4
Article
Ser(756) of β2 integrin controls Rap1 activity during inside-out activation of αMβ2
Lim J, Hotchin NA & Caron E (2011) Ser(756) of β2 integrin controls Rap1 activity during inside-out activation of αMβ2. Biochemical Journal, 437 (3), pp. 461-467. https://doi.org/10.1042/BJ20101666
Article
Rap1 controls activation of the α(M)β(2) integrin in a talin-dependent manner
Lim J, Dupuy AG, Critchley DR & Caron E (2010) Rap1 controls activation of the α(M)β(2) integrin in a talin-dependent manner. Journal of Cellular Biochemistry, 111 (4), pp. 999-1009. https://doi.org/10.1002/jcb.22788
Article
Four distinct structural domains in Clostridium difficile toxin B visualized using SAXS
Albesa-Jove D, Bertrand T, Carpenter EP, Swain GV, Lim J, Zhang J, Haire LF, Vasisht N, Braun V, Lange A, von Eichel-Streiber C, Svergun DI, Fairweather NF & Brown KA (2010) Four distinct structural domains in Clostridium difficile toxin B visualized using SAXS. Journal of Molecular Biology, 396 (5), pp. 1260-1270. https://doi.org/10.1016/j.jmb.2010.01.012
Article
Senis YA, Tomlinson MG, Ellison S, Mazharian A, Lim J, Zhao Y, Kornerup KN, Auger JM, Thomas SG, Dhanjal T, Kalia N, Zhu JW, Weiss A & Watson SP (2009) The tyrosine phosphatase CD148 is an essential positive regulator of platelet activation and thrombosis. Blood, 113 (20), pp. 4942-4954. https://doi.org/10.1182/blood-2008-08-174318
Article
An essential role for talin during alpha(M)beta(2)-mediated phagocytosis
Lim J, Wiedemann A, Tzircotis G, Monkley SJ, Critchley DR & Caron E (2007) An essential role for talin during alpha(M)beta(2)-mediated phagocytosis. Molecular Biology of the Cell, 18 (3), pp. 976-985. https://doi.org/10.1091/mbc.E06-09-0813
Article
Cougoule C, Hoshino S, Dart A, Lim J & Caron E (2006) Dissociation of recruitment and activation of the small G-protein Rac during Fcgamma receptor-mediated phagocytosis. Journal of Biological Chemistry, 281 (13), pp. 8756-8764. https://doi.org/10.1074/jbc.M513731200
Article
Wiedemann A, Patel JC, Lim J, Tsun A, van Kooyk Y & Caron E (2006) Two distinct cytoplasmic regions of the beta2 integrin chain regulate RhoA function during phagocytosis. Journal of Cell Biology, 172 (7), pp. 1069-1079. https://doi.org/10.1083/jcb.200508075
Book Chapter
Small GTP Binding Proteins and the Control of Phagocytic Uptake
Wiedemann A, Lim J & Caron E (2005) Small GTP Binding Proteins and the Control of Phagocytic Uptake. In: Rosales C (ed.) Molecular Mechanisms of Phagocytosis. Medical Intelligence Unit. New York: Landes Bioscience/Springer Science, pp. 72-84. http://link.springer.com/chapter/10.1007/978-0-387-28669-3_6#
Article
Phagocytosis, an alternative model system for the study of cell adhesion
Cougoule C, Wiedemann A, Lim J & Caron E (2004) Phagocytosis, an alternative model system for the study of cell adhesion. Seminars in Cell and Developmental Biology, 15 (6), pp. 679-689. https://doi.org/10.1016/j.semcdb.2004.09.001
Article
Mathieu R, Lim J, Simpson P, Prasannan S, Fairweather NF & Matthews S (2003) Resonance assignment and topology of a clostridial repetitive oligopeptide (CROP) region of toxin A from Clostridium difficile. Journal of Biomolecular NMR, 25 (1), pp. 83-84. https://doi.org/10.1023/A%3A1021990314599
I teach on the following modules:
1st Yr: BIOU1CB Introduction to Cell Biology (Module Co-ordinator) 2nd Yr: SCIU3CB Skills for Cell Biologists 3rd Yr: BIO6AC The Animal Cell (Module Co-ordinator) 3rd Yr: BIOU6AI Applied Immunology 4th Yr: BIOU9CP Applied Biological Sciences and Cell Biology Dissertations (Module Co-ordinator) In addition to that, I offer (on an ad-hoc basis):
Summer industrial placements for 2nd/3rd year UG students Projects for MSc: ENMPG18 Environmental Management (Conservation) Summer placements in my laboratory for the High School students through the Nuffield Research Placements Please contact me if you wish to undertake a project with me.