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Citation

Moresco V, Damazo NA & Barardi CRM (2016) Thermal and temporal stability on the enteric viruses infectivity in surface freshwater. Water Science and Technology: Water Supply, 16 (3), pp. 620-627. https://doi.org/10.2166/ws.2015.171

Abstract
The present study aimed to evaluate the stability of Human Adenovirus type 2 (HAdV2) and Murine Norovirus 1 (MNV-1) in surface freshwater samples stored at different temperatures. For HAdV2 the stability decreased with increasing temperatures (?80 > ?20 > 4 > 22 °C). The time required to reach one log reduction in viral titers (T90) was similar among all the times and temperatures by different cell-culture based methods and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The HAdV2 stability decreased with the time of storage temperature and methods employed, aside from samples stored at 22 and 4 °C which showed the lowest T90 values (50 days). For MNV-1, the samples stored at 22 and ?20 °C showed higher log10 decay values, followed by 4 and ?80 °C; while genome persistence was ranked as ?80 > ?20 > 4 > 22 °C. The T90 values were lower for samples stored at 22 °C (33 days), followed by 4, ?20 and ?80 °C with 111, 100 and 333 days, respectively. The results indicate that, under laboratory storage conditions, freshwater samples should be kept at 4 °C and at ?80 °C for short- and long-term periods, respectively. This study provided useful information about thermal and temporal stability of the enteric viruses regarding sample storage conditions.

Keywords
enteric viruses; freshwater; infectivity; storage temperature

Journal
Water Science and Technology: Water Supply: Volume 16, Issue 3