我要吃瓜

Citation

Varela ES, Bekaert M, Ganeco-Kirschnik LN, Torati LS, Shiotsuki L, de Almeida FL, Villela LCV, Rezende FP, da Silva Barroso A, de Freitas LEL, Taggart JB & Migaud H (2021) A high-density linkage map and sex-linked markers for the Amazon Tambaqui Colossoma macropomum. BMC Genomics, 22 (1), Art. No.: 709. https://doi.org/10.1186/s12864-021-08037-8

Abstract
Tambaqui (\textit{Colossoma macropomum}, Cuvier, 1818) is the most economically important native freshwater fish species in Brazil. It can reach a total length of over 1~m and a weight of over 40~kg. The species displays a clear sex dimorphism in growth performance with females reaching larger sizes at harvest. In aquaculture, the production of monosex populations in selective breeding programmes has been therefore identified as a key priority. In the present study, a genetic linkage map was generated by double digest restriction-site associated DNA (ddRAD) sequencing from 248 individuals sampled from two F1 families. The map was constructed using 14,805 informative SNPs and spanned 27 linkage groups. From this, the tambaqui draft genome was improved, by ordering the scaffolds into chromosomes, and sex-linked markers were identified. A total of 235 markers on linkage group 26 showed a significant association with the phenotypic sex supporting an XX/XY sex determination system in the species. The four most informative sex-linked markers were validated on another 206 sexed individuals demonstrating an accuracy in predicting sex ranging from 90.0\% to 96.7\%. The genetic mapping and novel sex-linked DNA markers identified and validated offer new tools for rapid progeny sexing, thus supporting the development of monosex female production in the industry while also supporting breeding programs of the species.

Keywords
Genetics; Biotechnology

Journal
BMC Genomics: Volume 22, Issue 1

• S12864-021-08037-8