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Citation

Watkins CA, Gaines T, Strathdee F, Baily JL, Watson E, Hall AJ, Free A & Dagleish MP (2022) A comparative study of the fecal microbiota of gray seal pups and yearlings ‐ a marine mammal sentinel species. MicrobiologyOpen, 11 (3), Art. No.: e1281. https://doi.org/10.1002/mbo3.1281

Abstract
Gray seals (Halichoerus grypus) can act as sentinel species reflecting the condition of the environment they inhabit. Our previous research identified strains of pathogenic Campylobacter and Salmonella, originating from both human and agricultural animal hosts, on rectal swabs from live gray seal (H. grypus) pups and yearlings on the Isle of May, Scotland, UK. We examined rectal swabs from the same pup (n?=?90) and yearling (n?=?19) gray seals to gain further understanding into the effects of age-related changes (pup vs. yearling) and three different natal terrestrial habitats on seal pup fecal microbiota. DNA was extracted from a subset of rectal swabs (pups n?=?23, yearlings n?=?9) using an optimized procedure, and the V4 region of the 16S ribosomal RNA gene was sequenced to identify each individual's microbiota. Diversity in pup samples was lower (3.92?±?0.19) than yearlings (4.66?±?0.39) although not significant at the p?=?0.05 level (p?=?0.062) but differences in the composition of the microbiota were (p?

Keywords
fecal microbiota; gray seal; pups; yearlings

Journal
MicrobiologyOpen: Volume 11, Issue 3

• MicrobiologyOpen - 2022 - Watkins - A Comparative Study Of The Fecal Microbiota Of Gray Seal Pups And Yearlings ? A Marine