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Citation

Caballero-Solares A, Hall JR, Xue X & Rise ML (2022) Reverse Transcription-Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) for Gene Expression Analyses. In: Christian SL (ed.) Cancer Cell Biology. Methods in Molecular Biology, 2508. Springer US, pp. 319-340. https://doi.org/10.1007/978-1-0716-2376-3_21

Abstract
The reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) is considered to be the gold standard for gene expression research. However, for this claim to be valid, RT-qPCR studies must test and optimize the quality of its RNA templates and assays. This chapter describes the experimental procedures required to generate reliable and reproducible gene expression results using RT-qPCR.