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Citation

Donate C, Balasch JC, Callol A, Bobe J, Tort L & MacKenzie S (2010) The effects of immunostimulation through dietary manipulation in the rainbow Trout; Evaluation of mucosal immunity. Marine Biotechnology, 12 (1), pp. 88-99. https://doi.org/10.1007/s10126-009-9203-4

Abstract
Immunostimulant-containing diets are commonly used in aquaculture to enhance the resistance of cultured fish to disease and stress. Although widespread in use, there have been conflicting results published, and surprisingly little is known about the regulation of immune response-related genes in tissues key to mucosal immunity induced by immunostimulant dietary feeding. Using a salmonid-specific microarray platform enriched with immune-related genes and in situ hybridization, we investigated dietary acclimation in two organs relevant to mucosal immunity, the gills and the intestine, in the rainbow trout (Oncorhynchus mykiss). Immunostimulant diets significantly changed gene expression profiles and gene distribution in a tissue-specific manner: genes and functional Gene Ontology categories involved in immunity were differently expressed at portals of entry where significant changes in genes and functional groups related to remodeling processes and antigen presentation were observed. Furthermore, genes involved in chemotaxis, cell differentiation, antigen-presenting capacity and tissue remodeling were localized in both organs.

Keywords
Immunostimulants; Oncorhynchus mykiss; Portals of entry; Microarray; Gene expression profiles

Journal
Marine Biotechnology: Volume 12, Issue 1