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Citation

Tocher DR, Bell JG & Sargent JR (1996) Induction of Delta 9-fatty acyl desaturation in rainbow trout (Oncorhynchus mykiss) liver by dietary manipulation. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 113 (1), pp. 205-212. https://doi.org/10.1016/0305-0491%2895%2902052-7

Abstract
Hepatic stearoyl Δ9-fatty acyl desaturation was demonstrated to be modulated in trout by dietary manipulation. Desaturation was very low in hepatocytes isolated from the livers of starved fish with only 2% of [1-14c]18:0 converted to Δ9-desaturase products (18:1n-9 and 20:1n-9). After 6 weeks of feeding a commercial (fish oil-containing) pelleted trout diet, 50% of the radioactivity from [1-14c]18:0 was converted to Δ9-desaturase products. Another period of starvation, after the 6-week feeding period, reduced Δ9-desaturation with only 4–5% of radioactivity from [1-14c]18:0 being converted to Δ9-desaturase products in hepatocytes. Subsequent refeeding produced a much more rapid induction of Δ9-desaturation with the activity significantly increased after only 3 days refeeding and the activity restored to the level before starvation after only 1 week of refeeding. The fatty acid composition of the diet appeared to be less important to the induction of hepatic Δ9-desaturation than the overall nutritional status because Δ9-desaturation in livers of fish fed a high saturated fatty acid diet, comprising 96% palmitic acid (16:0), was similar to that of fish fed a fish oil-containing diet.

Keywords
Oncorhynchus mykiss; trout; stearoyl Delta 9-desaturation; dietary manipulation; hepatocytes

Journal
Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology: Volume 113, Issue 1